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Collagen Fibers, a hierarchical material
Collagen is an important protein component in animal tissue structure and in manufactured materials. It is used in applications as varied as leather, gelatin, and artificial skin.
Collagen fibers are hierarchical materials. The basic structural unit is an individual collagen monomer. A collagen 'monomer' contains three proteins arranged in a triple helix with nonhelical ends. In type 1 collagen, monomers are about 280-300 nm long and 1.5 nm in diameter. Other types have different monomer lengths. Three types of collagen form large fibrillar structures: Type I, found in skin, tendon, and bone; Type II, found in cartilage; and Type III found in Skin.
Five monomers assemble into one microfibril which is about 4 nm in diameter. The monomers align parallel to one another but are staggered so that the end of one monomer is offset by about 67 nm from its neighbor. Many microfibrils associate to form collagen fibrils; in skin, the diameter can range from 30 to 300 nm. Covalent cross links repeat at regular intervals along the fiber following the fixed stagger pattern and bind all of the molecules in the fiber together. This leads to the "D periodic" banding that is one of the characteristic features of fibrillar collagen examined by TEM (transmission electron microscopy). Because the monomer length is about 4.4 to 4.5D, a fiber contains gaps where the ends of linearly arranged monomers do not meet. On the other hand, regions of side-by-side overlap contain more protein than gap regions. In TEM images made using traditional negative staining, the gaps trap more of the stain and thus appear darker.
In AFM, with no staining, the D period is detected as a distinct height variation along the fibril's length. In the example below, the height variation due to the D period was about 8% of the overall height of the fibril.
Historical note and References.
The images presented here some of the first Tapping Mode images of collagen fibers ever made. They were captured at Digital Instruments in June 1992, in a collaboration with Kevin Kjoller and Ellen Chernoff. Sample preparation is discussed in the papers below.
"Atomic Force Microscope Images of Collagen Fibers", Ellen A.G. Chernoff and Donald A. Chernoff, J. Vac. Sci. Technol. A 10, 596 (1992).
"Contact and non-contact Atomic Force Microscopy of Type I Collagen", Ellen A.G. Chernoff, Donald A. Chernoff, and Kevin Kjoller, Proc. 51st Ann. Mtg. of the Microscopy Society of America, p. 518, 1993.
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